Generation 1 cartoon
The Female Autobots were believed destroyed by a missile strike during the Ark's launch 4 million years ago.
By 1985, they were the last active resistance on Cybertron, operating in secret. Shockwave discovered their continued existence (thanks to Moonracer) and tracked them back to their underground headquarters. A subsequent attempt to capture Elita One resulted in a reunion with the Autobots of Earth, who believed the Female Autobots had perished long ago.
The Female Autobots relocated to a new secret headquarters provided by Alpha Trion.
They are then never seen or spoken of again. Not even by Arcee. It's THAT secret.
The two unnamed female Autobots seem to serve a more support/defensive role, while Chromia leads a strike force out on raids.
Seen manning a series of monitors in their original headquarters.
When trapped in their collapsed base, she appears more visibly defeated than any of her comrades. She transforms into a green Cybertronian car.
She seems to mix less with the others.
She's purple and orange, like Huffer. And she doesn't do much, like Huffer. The only member of Elita One's command not present in flashbacks to the Ark's launch, she's seen looking at a clipboard in their original base.
In contrast to her green comrade, she appears casual, even nonchalant while trapped in their ruined base. She transforms into a orange and purple Cybertronian car.